Affinity Chromatography (eBook, PDF)

Methods and Protocols
Redaktion: Zachariou, Michael

• Format: PDF

Produktbeschreibung

Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results. Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 348
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781597455824
• Artikelnr.: 64700408

Autorenporträt

Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results. Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.

Inhaltsangabe

Preface.- Contributors.- Affinity Chromatography: History, Perspectives, Limitations and ProspectsAna Ceclia A. Roque and Christopher R. Lowe 1.- Various Modes of Affinity Chromatography.- Immobilized Metal Ion Affinity Chromatography of Native ProteinsAdam Charlton and Michael Zachariou.- Affinity Precipitation of Proteins Using Metal ChelatesAshok Kumar, Igor Yu. Galaev, and Bo Mattiasson.- Immunoaffinity ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle.- Dye Ligand ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle.- Purification of Proteins Using Displacement ChromatographyNihal Tugcu.- Affinity Chromatography Using Purification Tags.- Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein LAna Ceclia A. Roque and Christopher R. Lowe.- Phage Display of Peptides in Ligand Selection for Use in Affinity ChromatographyJoanne L. Casey, Andrew M. Coley, and Michael Foley.- Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion ProteinGareth M. Forde.- Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion ProteinsAdam Charlton and Michael Zachariou.- Methods for the Purification of HQ-Tagged ProteinsBecky Godat, Laurie Engel, Natalie A. Betz, and Tonny M. Johnson.- Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding MethodsLeonard K. Pattenden and Walter G. Thomas.- Methods for Detection of Protein-Protein and Protein-DNA Interactions Using HaloTagMarjeta Urh, Danette Hartzell, Jacqui Mendez, Dieter H. Klaubert, and Keith Wood.- Site-Specific Cleavage of Fusion ProteinsAdam Charlton.- The Use of TAGZyme for the Efficient Removal of N-Terminal His-TagsJose? Arnau, ConniLauritzen, Gitte Ebert Petersen, and John Pedersen.- Various Applications of Affinity Chromatography.- Affinity Processing of Cell-Containing Feeds Using Monolithic Macroporous Hydrogels, CryogelsIgor Yu. Galaev and Bo Mattiasson.- Monolithic Bioreactors for MacromoleculesMojca Ben?ina, Katja Ben?ina, Ales Podgornik, and Ales Strancar.- Plasmid DNA Purification Via the Use of a Dual Affinity ProteinGareth M. Forde.- Affinity Chromatography of Phosphorylated ProteinsGrigoriy S. Tchaga.- Protein Separation Using Immobilized Phospholipid ChromatographyTzong-Hsien Lee and Marie-Isabel Aguilar.- Analysis of Proteins in Solution Using Affinity Capillary ElectrophoresisNiels H. H. Heegaard, Christian Schou, and Jesper Østergaard.

Rezensionen

From the reviews of the second edition:

"The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. ... recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields." (G. Ya. Wiederschain, Biochemistry, November, 2008)