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Analytical Ultracentrifugation , the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals. Timely contribution that describes a rapidly changing field | Leading researchers in the field | Broad coverage: instrumentation, basic theory, data analysis, and applications …mehr
Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software.
This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.
Timely contribution that describes a rapidly changing field
Leading researchers in the field
Broad coverage: instrumentation, basic theory, data analysis, and applications
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Inhaltsangabe
1. Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge Joseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen 2. Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation Data Gary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler 3. Sedimentation Velocity: A Classical Perspective John J. Correia and Walter F. Stafford 4. Hydrodynamic Modeling and its Application in AUC Mattia Rocco and Olwyn Byron 5. Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data Chad A. Brautigam 6. Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted and Crowded Solutions. Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas 7. Analysis of Linked Equilibria JiaBei Lin and Aaron L. Lucius 8. Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape Analysis of Sedimentation Velocity Data Catherine T. Chaton and Andrew B. Herr 9. Quaternary Structure Analyses of an Essential Oligomeric Enzyme Tatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini 10. Characterization of Intrinsically Disordered Proteins by Analytical Ultracentrifugation David J. Scott and Donald J. Winzor 11. Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and Fibrils Yee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin 12. AUC and Small Angle Scattering for Membrane Proteins Aline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel 13. Hydrodynamic Models of G-quadruplex Structures Jonathan B. Chaires, William L. Dean, Huy Le and John O. Trent 14. Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA Interactions Teng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf 15. Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy Ingrid Tessmer and Michael G. Fried 16. Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes Using Interference Detection. Hansen and Jeffrey 17. Methods in Enzymology - Analytical Ultracentrifugation David L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert 18. Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates and Lignins Stephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis 19. Analytical Ultracentrifugation and its Role in Development and Research of Therapeutical Proteins. Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire 20. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC Kelly K. Arthur, Brent S. Kendrick and John P. Gabrielson 21. Protein Assembly in Serum and the Differences from Assembly in Buffer. John J. Hill and Thomas M. Laue
1. Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge Joseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen 2. Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation Data Gary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler 3. Sedimentation Velocity: A Classical Perspective John J. Correia and Walter F. Stafford 4. Hydrodynamic Modeling and its Application in AUC Mattia Rocco and Olwyn Byron 5. Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data Chad A. Brautigam 6. Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted and Crowded Solutions. Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas 7. Analysis of Linked Equilibria JiaBei Lin and Aaron L. Lucius 8. Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape Analysis of Sedimentation Velocity Data Catherine T. Chaton and Andrew B. Herr 9. Quaternary Structure Analyses of an Essential Oligomeric Enzyme Tatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini 10. Characterization of Intrinsically Disordered Proteins by Analytical Ultracentrifugation David J. Scott and Donald J. Winzor 11. Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and Fibrils Yee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin 12. AUC and Small Angle Scattering for Membrane Proteins Aline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel 13. Hydrodynamic Models of G-quadruplex Structures Jonathan B. Chaires, William L. Dean, Huy Le and John O. Trent 14. Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA Interactions Teng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf 15. Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy Ingrid Tessmer and Michael G. Fried 16. Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes Using Interference Detection. Hansen and Jeffrey 17. Methods in Enzymology - Analytical Ultracentrifugation David L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert 18. Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates and Lignins Stephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis 19. Analytical Ultracentrifugation and its Role in Development and Research of Therapeutical Proteins. Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire 20. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC Kelly K. Arthur, Brent S. Kendrick and John P. Gabrielson 21. Protein Assembly in Serum and the Differences from Assembly in Buffer. John J. Hill and Thomas M. Laue
Rezensionen
Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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