This research developed a new method to measure bacterial growth potential (BGP) using the native bacterial consortium in seawater. New reagents to extract and detect ATP in microbial cells were specifically developed for seawater. The new lysis and detection reagents overcame the salt interference in seawater and allow low detection of total ATP, free ATP and microbial ATP in seawater. Incorporating a filtration step further increased the sensitivity of the method six fold, enabling ATP detection of ultra-low levels of microbial ATP in seawater.
The newly developed ATP-based BGP method was applied to monitor and assess the pre-treatment of five full-scale seawater desalination plants around the world. A good correlation was observed between BGP measured in SWRO feed water and the pressure drop increase in the SWRO systems, suggesting the applicability of using the ATP-based BGP method as a biofouling indicator in SWRO. Furthermore, a safe level of BGP (<70 µg/L) is proposed for SWRO feed water in order to ensure a chemical cleaning frequency of once/year or lower. However, to validate this conclusion, more SWRO plants with different pre-treatment systems need to be monitored.
In the future, on-line monitoring of BGP in SWRO feed water may further reduce the consumption of chemicals and energy and improve the overall sustainability of seawater desalination by reverse osmosis.
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