Biological Low-Voltage Scanning Electron Microscopy (eBook, PDF)

Redaktion: Pawley, James; Schatten, Heide

• Format: PDF

Produktbeschreibung

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It is the first book to focus on biological application of LVFESEM. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 317
• Erscheinungstermin: 3. Dezember 2007
• Englisch
• ISBN-13: 9780387729725
• Artikelnr.: 37286180

Inhaltsangabe

The Early Development of the Scanning Electron Microscope.- LVSEM for Biology.- The Aberration-Corrected SEM.- Noise and Its Effects on the Low-Voltage SEM.- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols.- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM.- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM.- Cryo-SEM of Chemically Fixed Animal Cells.- High-Resolution and Low-Voltage SEM of Plant Cells.- High-Resolution Cryoscanning Electron Microscopy of Biological Samples.- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy.

Rezensionen

From the reviews:

"Topics ranging from the early development of the scanning electron microscope (SEM) to some of the latest low-voltage and cryo techniques are reviewed in detail. ... this is a very useful volume for those wishing to learn, or refresh their memory, in the context of getting the best out of an SEM, and in particular the field emission SEM (FESEM)." (Iolo Ap Gwynn, infocus Magazine, Issue 12, December, 2008)
"Biological Low-voltage Scanning Electron Microscopy is an excellent and timely collection, written by many of the recognized experts in the subject ... . Strongly recommended." (Ultramicroscopy, Vol. 109, June, 2009)