Cryo-electron Tomography (eBook, ePUB)

A Journey From Sample Preparation to Data Mining
Redaktion: Hanein, Dorit; Volkmann, Niels

• Format: ePub

Produktbeschreibung

Cryo-Electron Tomography: A Journey from Sample Preparation to Data Mining providing a holistic overview of this rapidly advancing field and equipping researchers with the knowledge and tools necessary to initiate their own investigations. The book begins with a section on advanced cryogenic sample preparation for in situ cellular tomography, covering a range of sample types including viruses, bacteria, eukaryotic cells, and perspectives for organoids and tissues. Specific chapters focus on essential approaches such as vitrification, sample thinning, and bimodal correlative techniques. The book then transitions to a section on data mining and validation. - Offers a comprehensive overview of cryo-ET sample preparation and data mining protocols, consolidating approaches in a single resource - Includes foundational knowledge underpinning the field - Explores the latest methods, techniques and recent developments in cryo-ET - Offers guidance to researchers for key procedures - Written by an international range of experts

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 360
• Erscheinungstermin: 25. Juli 2025
• Englisch
• ISBN-13: 9780443188305
• Artikelnr.: 74880458

Inhaltsangabe

1. Characteristics of Sample Preparation for Electron Microscopy
2. Cryo-FIB Developments to Enable Nanoscale Biopsies
3. Preparing Bacterial Cells for Cryo-electron Tomography
4. Revealing the Hidden World of Microbes in Situ: Sample Preparation
Workflows for Cryo-electron Tomography
5. Mastering the Preparation of Eukaryotic Cell Samples for Cryo-ET: Tips
and Techniques
6. Interpretation of Cellular Tomograms
7. Denoising
8. Feature Detection in cryo-Electron Tomography Image Analysis
9. Validation