Downstream Processing of Proteins (eBook, PDF)

Methods and Protocols
Redaktion: Desai, Mohamed A.

• Format: PDF

Produktbeschreibung

Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 229
• Erscheinungstermin: 5. Februar 2008
• Englisch
• ISBN-13: 9781592590278
• Artikelnr.: 69699278

Inhaltsangabe

Downstream Processing in the Biotechnology Industry.- 2 Microbial Cell Disruption by High-Pressure Homogenization.- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration.- 4 High-Performance Tangential Flow Filtration for Protein Separations.- Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation.- Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules.- Application of Chromatography in the Downstream Processing of Biomolecules.- Affinity Ligand Technology.- Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules.- Expanded Bed Adsorption in the Purification of Biomolecules.- Application of HPLC in the Purification of Biomolecules.- Countercurrent Chromatography of Proteins with Polymer Phase Systems.- Practical Aspects and Applications of Radial Flow Chromatography.- Sterile Filtration and Virus Filtration.- Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products.- Validation of Sterilizing Filters in the Biotechnology Industry.

Rezensionen

"The contributors are almost exclusively directly and actively involved in practical process applications on a day-to-day basis, which adds considerable credibility to the overall presentation. ... the editor succeeds in his aim, producing a straightforward and uncomplicated directory of the integrated steps necessary to bring a product to market." - Chromatographia

"This valuable book is the result of collective work of mostly corporate authors with important experience in downstream processing of proteins in industrial and biotechnological environments....It may be good textbook in industrial biotechnology courses especially because of ample protocols given in this book....The simplicity and clarity of the chapters is contains could be also of a great advantage for food science, which would certainly need to have good bench book....I would recommend highly this unpretentious but highly valuable book also to the readers active in our field."-Nahrung/Food

"a worthwhile addition to the reference collections of special and academic libraries that serve scientists interested in the study and isolation of medical products from natural materials."-E-streams Electronic Book Reviews