Erythropoiesis (eBook, PDF)

Methods and Protocols
Redaktion: Lloyd, Joyce A.

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols for the study of erythropoiesis

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 298
• Erscheinungstermin: 26. Oktober 2017
• Englisch
• ISBN-13: 9781493974283
• Artikelnr.: 64700884

Inhaltsangabe

An Introduction to Erythropoiesis Approaches.- Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis.- Mouse Models of Erythropoiesis and Associated Diseases.- Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells.- Stress Erythropoiesis Model Systems.- Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models.- Functional Analysis of Erythroid Progenitors by Colony-Forming Assays.- Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands.- Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells.- Analysis of Erythropoiesis Using Imaging Flow Cytometry.- Flow Cytometric Analysis of Erythroblast Enucleation.- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure.- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples.- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples.- Genome Editing of Erythroid Cell Culture Model Systems.- In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood.- Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.- Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products.