Extracellular Vesicles (eBook, PDF)

Methods and Protocols
Redaktion: Kuo, Winston Patrick; Jia, Shidong

• Format: PDF

Produktbeschreibung

Includes cutting-edge techniques for the study of extracellular vesicles

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 446
• Erscheinungstermin: 21. August 2017
• Englisch
• ISBN-13: 9781493972531
• Artikelnr.: 64700893

Inhaltsangabe

Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine.- Red Blood Cells: A Source of Extracellular Vesicles.- Isolation of Extracelullar Vesicles by Ultracentrifugation.- Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles.- Paper-Based Devices for Isolation of Extracellular Vesicles.- Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis.- Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads.- Polymer-Based Purification of Extracellular Vesicles.- Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification.- Purification Protocols for Extracellular Vesicles.- Characterization of Extracellular Vesicles by Surface Plasmon Resonance.- Extracellular Vesicle Isolation and Analysis by Western Blotting.- Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis.- Characterization of Extracellular Vesicles by FlowCytometry.- Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC).- Multi-Surface Antigen Staining of Larger Extracellular Vesicles.- Microcapillary Chip-Based Extracellular Vesicle Profiling System.- Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy.- Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy.- In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase.- Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters.- Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples.- Extracellular Vesicle miRNA Detection Using Molecular Beacons.- Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling.- An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles.- Isolation of Extracellular Vesicles from Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits.- Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation.- Isolation of Extracellular Vesicles from Breast Milk.- An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis.- Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles.- Production and Characterization of Extracellular Vesicles in Malaria.- Isolation of Extracellular Vesicles from Stem Cells.- The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model.- Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine.- Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles.