Flow Cytometry Applications in Cell Culture (eBook, ePUB)

Redaktion: Al-Rubeai, Mohamed; Emery, A Nichol

• Format: ePub

Produktbeschreibung

This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells.

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Produktdetails

• Verlag: Taylor & Francis eBooks
• Seitenzahl: 344
• Erscheinungstermin: 24. Juli 2020
• Englisch
• ISBN-13: 9781000148503
• Artikelnr.: 59827071

Autorenporträt

Mohamed Al-Rubeai, A. Nicholas Emery

Inhaltsangabe

Monitoring the proliferative capacity of cultured animal cells by cell
cycle analysis; use of flow cytometry for monitoring antibody productivity
and isolating high-secreting hybridoma cells; antibody secretion assays
using gel microdrops and flow cytometry; high resolution cell cycle
analysis of cell cycle-regulated gene expression; stability of monoclonal
antibody production in hybridoma cell culture; flow cytometric studies of
osmotically stressed and sodium butyrate-treated hybridoma cells; flow
cytometric analysis of cells obtained from human bone marrow cultures;
measurement of intracellular pH during the cultivation of hybridoma cells
in batch and continuous cultures; the relationship between intracellular pH
and cell cycle in cultured animal cells using SNARF-1 indicator; assessment
of cell viability in mammalian cell lines; analysis of apoptosis by flow
cytometry; monitoring baculovirus infection and protein expression in
insect cells; use of protein distribution to analyze budding yeast
population structure and cell cycle progression; cell cycle analysis of
microorganisms; bacterial characterization by flow cytometry; assessment of
viability of bacteria by flow cytometry; advances in the flow cytometric
characterization of plant cells and tissues.