Gene Expression Profiling (eBook, PDF)

Redaktion: Shimkets, Richard A.

• Format: PDF

Produktbeschreibung

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 168
• Erscheinungstermin: 3. Februar 2008
• Englisch
• ISBN-13: 9781592597512
• Artikelnr.: 64692060

Inhaltsangabe

Technical Considerations in Quantitating Gene Expression
Richard A. Shimkets
Gene Expression Quantitation Technology Summary
Richard A. Shimkets
Standardized RT-PCR and the Standardized Expression Measurement Center
James C. Willey, Erin L. Crawford, Charles R. Knight, K. A. Warner, Cheryl A. Motten, Elizabeth A. Herness, Robert J. Zahorchak, and Timothy G. Graves
GeneCalling: Transcript Profiling Coupled to a Gene Database Query
Richard A. Shimkets
Invader Assay for RNA Quantitation
Marilyn C. Olson, Tsetska Takova, LuAnne Chehak, Michelle L. Curtis, Sarah M. Olson, and Robert W. Kwiatkowski
Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays
Jennifer Lescallett, Marina E. Chicurel, Robert Lipshutz, and Dennise D. Dalma-Weiszhausz
Amplified Differential Gene Expression Microarray, Zhijian J. Chen and Kenneth D. Tew
Suppression Subtractive Hybridization
Denis V. Rebrikov, Sejal M. Desai, Paul D. Siebart, and Sergey A. Lukyanov
Small Amplified RNA-SAGE
Catheline Vilain and Gilbert Vassart
Gene Expression Informatics
Martin D. Leach
Index