Gene Therapy Protocols (eBook, PDF)

Volume 2: Design and Characterization of Gene Transfer Vectors
Redaktion: Ledoux, Joseph

• Format: PDF

Produktbeschreibung

In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). This second volume of the new and completely revised third edition will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia. Readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 313
• Erscheinungstermin: 10. Mai 2008
• Englisch
• ISBN-13: 9781603272483
• Artikelnr.: 64701059

Inhaltsangabe

Purification of Retrovirus Particles Using Heparin Affinity Chromatography.- Scaleable Purification of Adenovirus Vectors.- Quantifying the Titer and Quality of Adenovirus Stocks.- Chromatography-Based Purification of Adeno-Associated Virus.- Spectroscopic Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery Systems.- Real-Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments.- Production of Lentiviruses Displaying "Early-Acting" Cytokines for Selective Gene Transfer into Hematopoietic Stem Cells.- Fiber-modified Adenoviruses for Targeted Gene Therapy.- PEGylated Adenovirus for Targeted Gene Therapy.- Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties.- Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes.- Reducing the Genotoxic Potential of Retroviral Vectors.- Evaluation of Promoters for Use in Tissue-Specific Gene Delivery.- Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cells.- Regulated Expression of Adenoviral Vectors-Based Gene Therapies.- Liver-Directed Gene Therapy Using the Sleeping Beauty Transposon System.- Generation and Functional Analysis of Zinc Finger Nucleases.- Conditional Gene Expression and Knockdown Using Lentivirus Vectors Encoding shRNA.- Nanoparticle-Mediated Gene Delivery to the Lung.- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype.- In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription.- Efficient Retroviral Gene Transfer to Epidermal Stem Cells.

Rezensionen

From the reviews of the third edition:

"This book reflects progress in the field with chapters on early stage vector development and in vitro evaluation, as well as others devoted to issues faced in preclinical and clinical development ... . Readers of this book are likely to be those working in vector development, validation and production as well as those involved in gene-based clinical trials. The specialized nature of this book means that it will primarily be used by experienced researchers ... ." (Christopher Ring, Microbiology Today, June, 2009)