Glucose Transport (eBook, PDF)

Methods and Protocols
Redaktion: Lindkvist-Petersson, Karin; Hansen, Jesper S

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 274
• Erscheinungstermin: 7. Dezember 2017
• Englisch
• ISBN-13: 9781493975075
• Artikelnr.: 64692445

Inhaltsangabe

Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris.- Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3.- Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies.- GLUT Characterization using Frog Xenopus laevis Oocytes.- Glucose Uptake in Heterologous Expression Systems.- Evaluating the Impact of GLUT Inhibition on Cellular Bioenergetics Using a Seahorse XF Analyzer.- Glucose Transport Activity Measured in Giant Vesicles.- Design, Synthesis, and Evaluation of GLUT Inhibitors.- Applying Microfluidic Systems to Study Effects of Glucose at Single Cell Level.- A Growth-Based Screening System for Hexose Transporters in Yeast.- Identification of Insulin Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique.- Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking.- Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay.- GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence.- Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes.- Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery.- Quantification of Cell Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeled Assay.- Tracking GLUT2 Translocation by Live Cell Imaging.- GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis.