Immunochemistry of Solid-Phase Immunoassay (eBook, PDF)

Redaktion: Butler, John E.

• Format: PDF

Produktbeschreibung

Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.

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Produktdetails

• Verlag: Taylor & Francis eBooks
• Seitenzahl: 336
• Erscheinungstermin: 16. August 2024
• Englisch
• ISBN-13: 9781000714500
• Artikelnr.: 72289541

Autorenporträt

Butler\, John E.

Inhaltsangabe

General Principles: Perspectives, Configurations, and Principles (J.E.
Butler). The Chemical Properties of Solid-Phases and Their Interaction With
Proteins (P.J. Tarcha). Immunochemical Considerations in the Analysis of
Data from Non-Competitive Solid-Phase Immunoassays (J.H. Peterman).
Photophysics of Heterogeneous Immunoassays (O.S. Khahil). Enzyme Systems
and Enzyme Conjugates for Solid-Phase ELISA (T.T. Ngo). Practical
Applications: Competitive, Noncompetitive and Multianalyte Immunoassays
(R.P. Ekins). Antigen Quantification: Measurement of Multivalent Antigens
by Solid-Phase Immunoassay (R.G. Hamilton). Immunoblotting: Membrane
Filters as the Solid Phase for Immunoassays (W.R. Brown, S.E> Dierks, J.E.
Butler, and J.M. Gershoni). Quantitation of Specific Antibodies: methods of
Expression, Standards, Solid-Phase Considerations, and Specific
Applications (J.E> Butler and R.G. Hamilton). Critical Issues and Special
Topics: The Specificity of Monoclonal Antibodies for Enzymes in Solution
vs. Immobilized on Solid-Phases (L. Djavadi-Ohaniance and b. Friguet).
Studies on the Covalent Attachment Antibodies to COntrolled Surfaces (P.D.
Mize, A. Naqui, C.M. O'Connell, J.N. Waller, M. Fesler, R.G. Myatich, and
W.E. Keating). The Application of Traditional Immunochemical Methods to
Evaluate the Performance of Capture Antibodies Immobilized on Microtiter
Wells (J.E. Butler, K.S. Joshi, and W.R. Brown). Considerations of the
Interpretation of the Specificity of Monoclonal Antibodies Determined in
Solid-Phase Immunoassays (F.J. Stevens). Monosized Particles as Solid-Phase
for Immunoassays: Applications and Immunochemistry (K. Nustad, E. Paus, and
O. Børmer). Enzyme Immunofiltration Assay for Human Creatine Kinase
Isoenzyme MB (F. Poulsen and O.J. Bjerrum). The Use of Peptides as
Solid-Phase Antigens (M. Lacroix, G. Dionne, M. Zrein, R.J. Dwyer, and R.J.
Chalifour). Streptavidin: Purification, Utility, and Use in Antibody
Immobilization (M. Suter). The Kinetics of Solid-Phase