Innate Immune Activation (eBook, PDF)

Methods and Protocols
Redaktion: de Nardo, Dominic; de Nardo, Christine M

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 251
• Erscheinungstermin: 24. November 2017
• Englisch
• ISBN-13: 9781493975198
• Artikelnr.: 64700241

Inhaltsangabe

Emerging concepts in innate immunity.- Bioinformatic Assessment of macrophage activation by the innate immune system.- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system.- Modeling primary human monocytes with the trans-differentiation cell line BLaER1.- Measurement of NF- B activation in TLR-activated macrophages.- Biochemical isolation of the myddosome from murine macrophages.- Generation of innate immune reporter cell lines using retroviral transduction.- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER).- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis.- Methods for detecting ASC and ASC speck formation.- Measuring innate immune responses to bacterial viability.- Methods to study cell swelling-induced inflammasome activation.- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy.- Quantitative proteomics of secreted proteins.- Simultaneous detection of cellular viability and interleukin-1beta secretion from single cells by ELISpot.- Detection and quantification of MAV aggregation via confocal imaging.