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Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in intermediate filament associated…mehr
Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers
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Inhaltsangabe
Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins 1. BioID Identification of Lamin-Associated Proteins Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux 2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae Petros Batsios, Irene Meyer and Ralph Gräf 3. Purification and Structural Analysis of LEM-Domain Proteins Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin 4. Purification and Structural Analysis of SUN and KASH Domain Proteins F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz 5. Purification of Lamins and Soluble Fragments of NETs Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer 6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins Jason M. Berk and Katherine L. Wilson Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins 7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins Bertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori 8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat- Shock Protein Chaperoning and Kinase Rescue Anastasia Mashukova, Radia Forteza and Pedro J. Salas 9. Purification of Protein Chaperones and their Functional Assays with Intermediate Filaments Ming Der Perng, Yu-Shan Huang and Roy A. Quinlan 10. Purification and Structural Analysis of Plectin and BPAG1e José A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda 11. Purification and Structural Analysis of Desmoplakin Hee-Jung Choi and William I. Weis 12. Degradation of the Intermediate Filament Family by Gigaxonin Pascale Bomont Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers 13. Functional and Genetic Analysis of Plectin in Skin And Muscle Günther A. Rezniczek, Lilli Winter, Gernot Walko, and Gerhard Wiche 14. Functional and Genetic Analysis of Epiplakin in Epithelial Cells Sandra Szabo, Karl L. Wögenstein and Peter Fuchs 15. In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure Christopher Arnette,Jennifer L. Koetsier, Paul Hoover, Spiro Getsios and Kathleen J. Green 16. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers and Cornified Envelope Precursor Proteins Veronika Boczonadi and Arto Määttä 17. Microtubule-Actin Crosslinking Factor 1: Domains, Interaction Partners and Tissue-Specific Functions Dmitry Goryunov and Ronald K. H. Liem 18. Functional and Genetic Analysis of Neuronal Isoforms of BPAG1 Anisha Lynch-Godrei and Rashmi Kothary 19. "Functional and Genetic Analysis of Spectraplakins in Drosophila Ines Hahn, Matthew Ronshaugen, Natalia Sánchez-Soriano and Andreas Prokop 20. Functional and Genetic Analysis of VAB-10 Spectraplakin in C. Elegans Christelle Gally, Huimin Zhang and Michel Labouesse Part IV: Functional and Genetic Analysis of Lamin-associated proteins 21. Tagged Chromosomal Insertion Site (TCIS) System, a Method to Study Lamina Associated Chromatin Jennifer C. Harr and Karen L. Reddy 22. Lamin Binding Proteins in Caenorhabditis Elegans Agnieszka Dobrzynska, Peter Askjaer and Yosef Gruenbaum 23. Pathologically-Relevant Prelamin A Interactions with Transcription Factors Arantza Infante and Clara I. Rodríguez 24. MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope Mohammed Hakim Jafferali, Ricardo A. Figueroa and Einar Hallberg 25. Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins Mayuri Sharma, Jeremy P. Kamil and Donald M. Coen
Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins 1. BioID Identification of Lamin-Associated Proteins Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux 2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae Petros Batsios, Irene Meyer and Ralph Gräf 3. Purification and Structural Analysis of LEM-Domain Proteins Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin 4. Purification and Structural Analysis of SUN and KASH Domain Proteins F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz 5. Purification of Lamins and Soluble Fragments of NETs Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer 6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins Jason M. Berk and Katherine L. Wilson Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins 7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins Bertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori 8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat- Shock Protein Chaperoning and Kinase Rescue Anastasia Mashukova, Radia Forteza and Pedro J. Salas 9. Purification of Protein Chaperones and their Functional Assays with Intermediate Filaments Ming Der Perng, Yu-Shan Huang and Roy A. Quinlan 10. Purification and Structural Analysis of Plectin and BPAG1e José A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda 11. Purification and Structural Analysis of Desmoplakin Hee-Jung Choi and William I. Weis 12. Degradation of the Intermediate Filament Family by Gigaxonin Pascale Bomont Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers 13. Functional and Genetic Analysis of Plectin in Skin And Muscle Günther A. Rezniczek, Lilli Winter, Gernot Walko, and Gerhard Wiche 14. Functional and Genetic Analysis of Epiplakin in Epithelial Cells Sandra Szabo, Karl L. Wögenstein and Peter Fuchs 15. In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure Christopher Arnette,Jennifer L. Koetsier, Paul Hoover, Spiro Getsios and Kathleen J. Green 16. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers and Cornified Envelope Precursor Proteins Veronika Boczonadi and Arto Määttä 17. Microtubule-Actin Crosslinking Factor 1: Domains, Interaction Partners and Tissue-Specific Functions Dmitry Goryunov and Ronald K. H. Liem 18. Functional and Genetic Analysis of Neuronal Isoforms of BPAG1 Anisha Lynch-Godrei and Rashmi Kothary 19. "Functional and Genetic Analysis of Spectraplakins in Drosophila Ines Hahn, Matthew Ronshaugen, Natalia Sánchez-Soriano and Andreas Prokop 20. Functional and Genetic Analysis of VAB-10 Spectraplakin in C. Elegans Christelle Gally, Huimin Zhang and Michel Labouesse Part IV: Functional and Genetic Analysis of Lamin-associated proteins 21. Tagged Chromosomal Insertion Site (TCIS) System, a Method to Study Lamina Associated Chromatin Jennifer C. Harr and Karen L. Reddy 22. Lamin Binding Proteins in Caenorhabditis Elegans Agnieszka Dobrzynska, Peter Askjaer and Yosef Gruenbaum 23. Pathologically-Relevant Prelamin A Interactions with Transcription Factors Arantza Infante and Clara I. Rodríguez 24. MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope Mohammed Hakim Jafferali, Ricardo A. Figueroa and Einar Hallberg 25. Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins Mayuri Sharma, Jeremy P. Kamil and Donald M. Coen
Rezensionen
Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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