Laboratory Methods in Enzymology: Protein Part B (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on protein

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 240
• Erscheinungstermin: 24. Februar 2014
• Englisch
• ISBN-13: 9780124201798
• Artikelnr.: 40999999

Inhaltsangabe

Small-scale expression of proteins in E. coli
Protein expression-Yeast
Recombinant protein expression in baculovirus-infected insect cells
Single cell cloning of a stable mammalian cell line
In vitro synthesis of proteins in bacterial extracts
Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
Yeast two-hybrid screen
UV cross-linking of interacting RNA and protein in cultured cells
Analysis of RNA-protein interactions by cell mixing
General protein-protein cross-linking
Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
Dissecting a known RNA-protein interaction using a yeast three-hybrid system
Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system