K V Krishnamurthy

Methods in Cell Wall Cytochemistry (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.

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Produktdetails

• Verlag: Taylor & Francis eBooks
• Seitenzahl: 336
• Erscheinungstermin: 24. Juli 2020
• Englisch
• ISBN-13: 9781000144154
• Artikelnr.: 59827082

Autorenporträt

Krishnamurthy, K V

Inhaltsangabe

Preface Cell Wall-Morphology & Chemical Composition Carbohydrates Cellulose
Callose and other 1
3-b-glucans
Chitins
Pectins
Hemicelluloses
Alginic Acid
Sulphated Polysaccharides Glycoproteins Structural Proteins-Lectins
Arabinogalacton Rich Proteins (AGP's)
Hydroxy Proline Rich Proteins
Proline Rich Proteins
Glycine Rich Proteins
Threonine Rich Proteins
Thionins
Pistil Specific Extensin-Like Proteins Enzymes-Peroxidase
Polyamineoxidase
Phosphatase
Protease
Nitrate Reductase
Malate Dehydrogenase
Proline Hydroxylase
Glycosylhydrolases and Transferases
Non-Specific Esterases
and Ribonucleuses Lipids and Related Substances-Cutin
Suberin
Waxes
Sporopollenin
Pollenkitt
and Tryphine Lignin and Other Phenolic Substances-Lignins
Phenolic Acids and Amides Mineral Substances-Silica
Calcium
and Boron Light Microscopic Cytochemistry Introduction Tissue Preparation-Use of Fresh Living Tissue
Use of Frozen Tissue
Freeze Substitution and Drying
Chemical Fixation
Dehydration and Clearing
Infiltration and Embedding
Sectioning of Materials Stains and Staining-Introduction
Control Procedures
Incubation of Materials Mounting of Stained Materials Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing
Freeze Substitution and Drying Methods
Preparation of Fixatives
Dehydration and Clearing Procedures Procedures for the Cytochemical Localizations of Different Cell Wall Substances Carbohydrates-Insoluble Polysaccharides
Cellulose
Chitin
Callose
Sulphated and Carboxylated polysaccharides Proteins-Non-Specific Cell Wall Proteins
Enzymic Proteins Lignins Sporopollenins Cutins & Suberin Minerals Fluorescent Microscopic Cytochemistry Introduction Fluorescent Cytochemical Methods Cell Wall Carbohydrates Cell Wall Proteins Lignin Ferulic Acid Sporopollenin Cutin and Suberin Transmission Electron Microscopic Cytochemistry Introducti