Microinjection and Transgenesis (eBook, PDF)

Strategies and Protocols
Redaktion: Cid-Arregui, Angel; Garcia-Carranca, Alejandro

• Format: PDF

Produktbeschreibung

The most potent technique to study cell function is the specific tranfer of genetic material into a single cell. Several functions can than be analyzed in the resulting transgenic cell or organism. The method of microinjection and transgenesis is currently used in many cell biological laboratories.

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Produktdetails

• Verlag: Springer Berlin Heidelberg
• Seitenzahl: 619
• Erscheinungstermin: 6. Dezember 2012
• Englisch
• ISBN-13: 9783642803437
• Artikelnr.: 53087714

Inhaltsangabe

1 Strategies and Protocols for Microinjection Experiments Using Somatic Cells in Culture.- 1 Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells.- 2 Automated Computer-Assisted Microinjection into Cultured Somatic Cells.- 3 Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells.- 4 Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture.- 5 Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Experiments.- 6 Colorimetric in Situ Hybridization.- 7 Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tissue Culture Cells.- 8 Cellular Microbiochemistry.- 9 A New Strategy for Studying Microtubule Transport and Assembly During Axon Growth.- 10 The Use of Microinjection to Study Signal Transduction in Mammalian Cells.- 2 Strategies and Protocols for Microinjection Experiments Using Embryos.- 11 Supply and Husbandry of Mice for Transgenic Science.- 12 Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis?.- 13 Sperm-Mediated Gene Transfer.- 14 Application of Ligand-Dependent Site-Specific Recombination in ES Cells.- 15 A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryos.- 16 The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem Cells.- 17 The Generation of Transgenic Mice with Yeast Artificial Chromosomes.- 18 Following Up Microinjected mRNAs: Analysis of mRNA Poly(A) Tail Length During Mouse Oocyte Maturation.- 19 Mouse Preimplantation Embryos and Oocytes as an In Vivo System to Study Transcriptional Enhancers.- 20 The Use of Mouse Preimplantation Embryos for the Identification of DNA-Binding Proteins Essential in Early Development.- 21 Application of Transgenes and Transgenic Mice to Study Gene Activity from the Oocyte to the Early Embryo.- 22 Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements.- 23 Transgenic Analysis of Embryonic Gene Expression Using LacZ as a Reporter.- 24 Autonomous Cell Labeling Using a laacZ Reporter Transgene to Produce Genetic Mosaics During Development.- 25 Multiple Developmental Functions of the Zinc Finger Gene Krox-20 Are Revealed by Targeted Insertion of the E. coli lacZ Coding Sequence.- 26 Transgenic Mouse Strategies in Virus Research.- 27 Isolation of Mutated Genes from Transgene Insertion Sites.- 28 Contributions of Transgenic and Knockout Mice to Immunological Knowledge.- 29 Generation and Application of Transgenic Rabbits.- 30 Microinjection of Drosophila Eggs.