Multiple Myeloma (eBook, PDF)

Methods and Protocols
Redaktion: Brown, Ross D.; Ho, P. Joy

• Format: PDF

Produktbeschreibung

Although there is no therapy that could be considered curative for patients with multiple myeloma, recent research has gained considerable momentum and yielded a real prospect for achieving a significant improvement in response to therapy. In Multiple Myeloma: Methods and Protocols, established researchers with hands-on experience detail a comprehensive set of classic and innovative methodologies used in many laboratories for the investigation of this disease. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology, which is used routinely in many laboratories around the world to determine prognosis, to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Multiple Myeloma: Methods and Protocols offers both novice and experienced researchers alike a gold-standard collection of proven techniques for investigating the pathogenesis of multiple myeloma and identifying new therapeutic targets.

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Produktdetails

• Verlag: Humana Press
• Seitenzahl: 310
• Erscheinungstermin: 1. Februar 2008
• Englisch
• ISBN-13: 9781592599165
• Artikelnr.: 64616048

Inhaltsangabe

Multiple Myeloma: Challenges and Opportunities
Douglas Joshua, Ross D. Brown, and P. Joy Ho
Immunophenotyping of Plasma Cells in Multiple Myeloma
Gema Mateo Manzanera, Jesús F. San Miguel Izquierdo, and Alberto Orfao de Matos
Plasma Cell Labeling Index
Philip R. Greipp and Shaji Kumar
Conventional Cytogenetics in Myeloma
Lynda J. Campbell
Multicolor Spectral Karyotyping in Multiple Myeloma
Jeffrey R. Sawyer
Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization
Scott VanWier and Rafael Fonseca
Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma
Pulivarthi H. Rao
Southern Blotting of IgH Rearrangements in B-Cell Disorders
Edna Yuen and Ross D. Brown
Identification and Assembly of V Genes As Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma
Surinder S. Sahota, Mark Townsend, and Freda K. Stevenson
Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma
Brian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, and Linda M. Pilarski
Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma
Mara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, and Marco Ladetto
Incomplete DJH Rearrangements
David González and Ramón Garcia-Sanz
Identification of Malignant Plasma Cells by mRNA In Situ Hybridization
Ross D. Brown and P. Joy Ho
The SCID-hu Myeloma Model
Joshua Epstein and Shmuel Yaccoby
The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysis
Karin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik De Raeve, Pedro Couck, Frans Gorus, Peter Croucher, and Ben Van Camp
Determination of Telomerase Activity and Telomere Length
Kai-da Wu and Malcolm A. S. Moore
Dendritic Cell/Myeloma Hybrid Vaccine
Dajing Xia, Tim Chan, and Jim Xiang
Genetically Engineered Myeloma Cell Vaccine
Siguo Hao, Tim Chan, and Jim Xiang
An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors
Andrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, and Gerald J. Atkins
Clonality Detection of the Expanded T-Cell Populations in Patients With Multiple Myeloma
Daniel M-Y. Sze
Detecting Mismatch Repair Defects in Myeloma
Mark Velangi, Elizabeth Matheson, Andrew Hall, and Julie Irving
Methylation-Specific Polymerase Chain Reaction
Oliver Galm and James G. Herman
Making Sense of DNA Microarray Data
Wei-min Liu, Sunhee K. Ro, and Walter H. Koch
Index

Rezensionen

From the reviews:

"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." -- The Journal of the Association of Genetic Technologists

"Audience: Hematologists, oncologists, and other clinicians ... as well as biomedical researchers in the field of hematology and immunology. ... The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. ... covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics." (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)