Next Generation Sequencing (eBook, PDF)

Methods and Protocols
Redaktion: Head, Steven R.; Salomon, Daniel R.; Ordoukhanian, Phillip

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 266
• Erscheinungstermin: 9. Dezember 2017
• Englisch
• ISBN-13: 9781493975143
• Artikelnr.: 64701940

Inhaltsangabe

An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate in vivo Translatome.- Measuring Nascent Transcripts by Nascent-seq.- Genome-wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis.- Multiplexed Targeted Sequencing for Oxford Nanopore MinION; A Detailed Library Preparation Procedure.- Hi-Plex for Simple, Accurate, and Cost-effective Amplicon-based Targeted DNA Sequencing.- ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras.- Genome-wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach.- Sequencing of Genomes from Environmental Single Cells.- SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms.- CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers.- Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities.- A Bloody Primer Analysis of RNA-Seq from Tissue Admixtures.- Next-Generation Sequencing of Genome-wide CRISPR Screens.- Gene Profiling and T Cell Receptor Sequencing from Antigen-specific CD4 T Cells.- Investigate Global Chromosomal Interaction by Hi-C in Human Naïve CD4 T Cells.- Primer Extension, Capture, and On-bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites.