PCR Technology (eBook, PDF)

Current Innovations, Third Edition
Redaktion: Nolan, Tania; Bustin, Stephen A.

• Format: PDF

Produktbeschreibung

PCR's simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology.

Topics in this forward-thinking volume include:

• The purification and handling of PCR templates
• The effect of the manufacture and purification of the oligonucleotide on PCR behavior
• Optimum buffer composition
• Probe options
• The design and optimization of qPCR assays
• Issues surrounding the development and refinement of instrumentation
• Effective controls to protect against uncertainties due to reaction variability

Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.

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Produktdetails

• Verlag: Taylor & Francis eBooks
• Seitenzahl: 457
• Erscheinungstermin: 13. Juni 2013
• Englisch
• ISBN-13: 9781439848135
• Artikelnr.: 39042605

Autorenporträt

Dr. Tania Nolan is an internationally recognized molecular biologist with a reputation for expertise in the field of mRNA quantification using RT-qPCR. She was a significant coauthor of the specialist textbook The A-Z of Quantitative PCR along with numerous other book chapters and peer-reviewed papers. She has presented several plenary lectures and chaired sessions at major international qPCR meetings. Stephen A. Bustin, BS(Mod) PhD FSB acquired his first qPCR instrument in 1997 and has published numerous peer-reviewed papers that describe and use this technology. He wrote and edited the A-Z of Quantitative PCR, universally acknowledged as the "qPCR Bible," edited The PCR Revolution, and has written a series of Definitive qPCR ebooks. He also led the international consortium that drew up the MIQE guidelines.

Inhaltsangabe

Template Preparation. Pre-PCR Processing Strategies. Use of a Liposomal
Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic
Acid Amplification. Reaction Components. Oligonucleotide Synthesis and
Purification. Augmenting PCR: Effects of Additives in the Polymerase Chain
Reaction. Real-Time Fluorescent PCR by Labeled Primer with a Single
Fluorescent Molecule. Using Scorpion Primers for Genotyping. LNA: Adding
New Functionality to PCR. Modified dNTPs: A Toolbox for Use in PCR.
Instruments. Thermocycler Calibration and Analytical Assay Validation.
Ultra-High-Speed PCR Instrument Development. Dropletization of
Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene
Measurement and Cancer Diagnosis. Design, Optimization, QC, and
Standardization. Assay Design for Real-Time qPCR. Primer Design for
Large-Scale Multiplex PCR and Arrayed Primer Extension. Development and Use
of qPCR Assays for Detection and Study of Neglected Tropical and Emerging
Infectious Diseases. MIQE: Guidelines for Reliable Design and Transparent
Reporting of Real-Time PCR Assays. Data Analysis. Hypothesis-Driven
Approaches to Multivariate Analysis of qPCR Data. Experiment Design, Data
Management, and Univariate Statistical Analysis of Gene-Expression Data
Obtained by Real-Time Quantitative PCR. Biomarker Discovery via RT-qPCR and
Bioinformatical Validation. Optimization of Quantitative Real-Time PCR for
Studies in Cartilage Mechanobiology. Applications. Enriching DNA Sequences
with Nucleotide Variation by Thymidine Glycosylases Combined with
Suppression PCR. Chromosome Walking by Inverse PCR. Expression Profiling of
MicroRNAs by Quantitative Real-Time PCR: The Good, the Bad, and the Ugly.
Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark(TM).
Surface Plasmon Resonance-Based Biosensor Technology for Real-Time
Detection of PCR Products. Current Innovations in the Development and
Application of the qPCR in a Resource-Limited Setting. A Review of
Isothermal Nucleic Acid Amplification Technologies. Proximity Ligation
Assay for Protein Quantification. High-Resolution Melt Analysis. Index.