Phenotyping of Human iPSC-derived Neurons (eBook, ePUB)

Patient-Driven Research
Redaktion: Buttermore, Elizabeth D.

• Format: ePub

Produktbeschreibung

Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology to better understand neurological disorders and identify novel therapeutics, also providing considerations and best practices. By presenting example projects that identify phenotypes and mechanisms relevant to autism spectrum disorder and epilepsy, this book allows readers to understand what considerations are important to assess at the start of project design. Sections address reproducibility issues and advances in technology at each stage of the pipeline and provide suggestions for improvement. From patient sample collection and proper controls to neuronal differentiation, phenotyping, screening, and considerations for moving to the clinic, these detailed descriptions of each stage of the pipeline will help everyone, regardless of stage in the pipeline.

In recent years, drug discovery in the neurosciences has struggled to identify novel therapeutics for patients with varying indications, including epilepsy, chronic pain, and psychosis. Current treatment options for such patients are decades old and offer little relief with many side effects. One explanation for this lull in novel therapeutics is a lack of novel target identification for neurological disorders (and target identification requires exemplar preclinical data). To improve on the preclinical work that often relies on rodent modeling, the field has begun utilizing patient-derived induced pluripotent stem cells (iPSCs) to differentiate neurons in vitro for preclinical characterization of neurological disease and target identification.

• Discusses techniques and new technology for iPSC culturing and neuronal differentiation to establish best practices in the lab
• Outlines considerations for phenotypic assay development
• Provides information about the successes, failures, and implications of phenotyping and screening with iPSC-derived neurons
• Describes how human iPSC-derived neurons are being used for preclinical discovery research as well as the development of therapeutics utilizing hiPSC-derived neurons

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 372
• Erscheinungstermin: 9. September 2022
• Englisch
• ISBN-13: 9780128222782
• Artikelnr.: 65691812

Inhaltsangabe

Section I: Best practices and considerations when designing a new project
1. iPSC culture: best practices from sample procurement to reprogramming and differentiation
2. Phenotypic assay development with iPSC-derived neurons: technical
3. Derivation of cortical interneurons from human pluripotent stem cells to model neurodevelopmental disorders
4. Development of transcription factor-based strategies for neuronal differentiation from pluripotent stem cells
5. Differentiation of Purkinje cells from pluripotent stem cells for disease phenotyping in vitro
6. Brain organoids: models of cell type diversity, connectivity, and disease phenotypes

Section II: The use of iPSC-derived neurons to study neurological disorders
7. Human models as new tools for drug development and precision medicine
8. Use of cerebral organoids to model environmental and gene x environment interactions in the developing fetus and neurodegenerative disorders
9. iPSC-derived models of autism: Tools for patient phenotyping and assay-based drug discovery
10. Probing the electrophysiological properties of patient-derived neurons across neurodevelopmental disorders
11. Advantages and limitations of hiPSC-derived neurons for the study of neurodegeneration

Section III: New technology, industry perspective, and transitioning to the clinic
12. Developing clinically translatable screens using iPSC-derived neural cells
13. Gene editing hPSCs for modeling neurological disorders
14. Cell therapy and biomanufacturing using hiPSC-derived neurons
15. Ethical considerations for the use of stem cell-derived therapies