Plant Protein Secretion (eBook, PDF)

Methods and Protocols
Redaktion: Jiang, Liwen

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 285
• Erscheinungstermin: 31. August 2017
• Englisch
• ISBN-13: 9781493972623
• Artikelnr.: 64701136

Inhaltsangabe

An Overview of Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatics Analysis of Protein Secretion in Plants.- Proteomics Analysis of Secreted Proteins from Cell Microenvironment.- Using Homology Modelling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a/AtSec23a Pair Involved in Protein ER Export.- Analysis of Golgi-Mediated Protein Traffic in Plant Cells.- Analysis of Membrane Protein Topology in the Plant Secretory Pathway.- Semi-Automatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod.- 3D Printing of Plant Golgi Stacks from their Electron Tomographic Models.- Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics.- Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes.- Analysis of Phragmoplast Kinetics during Plant Cytokinesis.- Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells.- In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM).- Analysis of Nanobody-Epitope Interactions in Living Cells via Quantitative Protein Transport Assays.- A Secretion System for Identification of Cargo Proteins of Vacuolar Sorting Receptors.- Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination.- Measuring Plant Protein Secretion.- Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion.- The Organelle pH Estimate and Measurement in Plant Secretory Pathway.- Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion in Plant Cells.- Isolation of the Plant Exocyst Complex.- Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells.- Analysis of Plant Autophagy.