Polyamines (eBook, PDF)

Methods and Protocols
Redaktion: Alcázar, Rubén; Tiburcio, Antonio F.

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 512
• Erscheinungstermin: 27. Oktober 2017
• Englisch
• ISBN-13: 9781493973989
• Artikelnr.: 64701788

Inhaltsangabe

Polyamines in the Context of Metabolic Networks.- Molecules for Sensing Polyamines and Transducing their Action in Plants.- Polyamine Metabolism Responses to Biotic and Abiotic Stress.- Thermospermine: An Evolutionary Ancient but Functionally New Compound in Plants.- Determination of Polyamines by Capillary Electrophoresis using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent.- Detection of Thermospermine and Spermine by HPLC in Plants.- Development of Amine-Oxidase Based Biosensors for Spermine and Spermidine Analysis.- Identification of Branched-Chain Polyamines in Hyperthermophiles.- Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry.- Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-Phthalaldehyde.- Determination of Arginine and Ornithine Decarboxylase Activities in Plants.- Determination of S-Adenosylmethionine Decarboxylase Activity in Plants.- Determination of Copper Amine Oxidase Activity in Plant Tissues.- Determination of di-/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay.- Pentamine as a Substrate for Measuring Spermine Oxidase Activity.- Spectrophotometric Quantification of Reactive Oxygen, Nitrogen and Sulfur Species in Plant Samples.- Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay.- Determination of Transglutaminase Activity in Plants.- Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy during Organogenic Nodule Formation in Hop.- Analysis of the Intracellular Localization of Transiently Expressed and Fluorescently Labeled Copper-Containing Amine Oxidases, Diamine Oxidase and N-Methylputrescine Oxidase in Tobacco, using an Agrobacterium infiltration Protocol.- Techniques used for Functional Characterization of Polyamine Transporters.- Quantitative Trait Loci for RootGrowth Response to Cadaverine in Arabidopsis.- Methods Related to Polyamine Control of Cation Transport across Plant Membranes.- Analysis of DNA Methylation Content and Patterns in Plants.- Investigating Ornithine Decarboxylase Post-Transcriptional Regulation via a Pulldown Assay using Biotinylated Transcripts.- Analysis of Cotranslational Polyamine Sensing during Decoding of ODC Antiyme mRNA.- Modulation of Protein Synthesis by Polyamines in Mammalian Cells.- Determination of Post-Translational Modifications by 2D-PAGE: Applications to Polyamines.- Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine Genetics.- Transcriptome Analysis of PA Gain and Loss of Function Mutants.- High-Throughput Phenotyping in Plant Stress Response: Methods and Potential Applications to Polyamine Field.- Abiotic Stress Phenotyping of Polyamine Mutants.- Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes during Pathogenic Interactions.- Real-Time In Vivo Monitoring of Reactive Oxygen Species in Guard Cells.- Genome-Wide Association Mapping Analyses Applied to Polyamines .- Polyamine Metabolism in Climacteric and Non-Climacteric Fruit Ripening.- Application of Polyamines to Maintain Functional Properties in Stored Fruits.- Acrolein: An Effective Biomarker for Tissue Damage Produced from Polyamines.- Polyamines and Cancer.- Potential Applications of Polyamines in Agriculture and Plant Biotechnology.