Preeclampsia (eBook, PDF)

Methods and Protocols
Redaktion: Murthi, Padma; Vaillancourt, Cathy

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 355
• Erscheinungstermin: 30. November 2017
• Englisch
• ISBN-13: 9781493974986
• Artikelnr.: 64692000

Inhaltsangabe

Diagnostic Imaging: Ultrasound.- Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the Cobas e 411 Analyzer.- Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia.- Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia.- Genetic Approaches in Pre-Eclampsia.- Epigenetics and Pre-Eclampsia: Programming of Future Outcomes.- Inflammatory and Immune System Markers.- Methods to Enrich Exosomes from Conditioned Media and Biological Fluids.- Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants.- Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation.- Proteomics Method to Identification of Protein Profiles in Exosomes.- Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental Explants.- Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index.- Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects.- Immunohistological Techniques.- Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin.- Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta.- Analyzing Trophoblast Function Using Cell-Based Assays.- Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis.- An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro.- In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases.- Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System.- Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis.- Placental Lipid Transport.- EG-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal Model.- Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-Eclampsia.- Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-Eclampsia.- A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP Trial.