Prions (eBook, PDF)

Methods and Protocols
Redaktion: Lawson, Victoria A.

• Format: PDF

Produktbeschreibung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 371
• Erscheinungstermin: 31. August 2017
• Englisch
• ISBN-13: 9781493972449
• Artikelnr.: 64702012

Inhaltsangabe

Purification and Fibrillation of Full-length Recombinant PrP.- Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure.- Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy.- Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy.- Immunodetection of PrP^Sc using Western Immunoblotting Techniques.- Analysis of miRNA Signatures in Neurodegenerative Prion Disease.- Expression of Heterologous PrP and Prion Propagation in RK13 Cells.- Generation of Infectious Prions and Detection with the Prion Infected Cell Assay.- Analysis of Cellular Prion Protein Endoproteolytic Processing.- Cellular Analysis Adult Neural Stem Cells for Investigating Prion Biology.- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons.- Methods of Protein Misfolding Cyclic Amplification.- RT-QuIC Assays for Prion Disease Detection and Diagnostics.- A Quick Method to Evaluate the Effect of the Amino Acid Sequence inthe Misfolding Proneness of the Prion Protein.- Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases.- In vivo Near Infrared Imaging of Neurodegeneration.- Strain Typing of Prion Diseases using in vivo Mouse Models.- Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice.- Cell Culture Methods for Screening of Prion Therapeutics.- Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease.- Methods for Molecular Diagnosis of Human Prion Disease.- Molecular sub-typing of PrP^res in Human Sporadic CJD Brain Tissue.- Intercellular Prion-like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture.