Protocols for Macroalgae Research (eBook, ePUB)

Redaktion: Charrier, Bénédicte; Reddy, C R K; Wichard, Thomas

• Format: ePub

Produktbeschreibung

This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.

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Produktdetails

• Verlag: Taylor & Francis eBooks
• Seitenzahl: 518
• Erscheinungstermin: 17. April 2018
• Englisch
• ISBN-13: 9781351132930
• Artikelnr.: 56840129

Autorenporträt

Dr. Bénédicte Charrier is a senior scientist from CNRS working at Station Biologique Roscoff, France. She is a specialist of macroalgae morphogenesis and development, with an expertise on the filmentous alga Ectocarpus siliculosus. She is currently the Chair of the European network "Phycomorph", supported by the Cooperation in Science & Technology (COST) Association (2015-2019), which aims to coordinate research in growth, reproduction, and morphogenesis of macroalgae in Europe and in association with Asian and North American laboratories. Dr. Thomas Wichard is a research group leader at the Institute for　Inorganic and Analytical Chemistry of the Friedrich Schiller　University Jena. The main focus of his research group is to elucidate　the mutualistic interactions between bacteria and the marine macroalga　Ulva ("cross-kingdom-cross-talk"). The group applies various　methodologies in analytical chemistry, chemical ecology and molecular　biology to understand the basis of eco-physiological processes. Dr. CRK Reddy is a Chief Scientist in Seaweed Biology and Biotechnology Cultivation at CSIR-Central Salt and Marine Chemicals Research Institute in Bhavnagar, India. He is an expert in marine macroalgae, making significant contributions to macroalgae tissue culture and protoplast techniques including genetic improvement of seaweeds, seaweed biomass for chemicals and biofuels, seaweed biodiversity, and seaweed biology and cultivation.

Inhaltsangabe

CONTENTS
Section I: Cultivating and preserving seaweeds
Chapter 1 Seaweed in high-energy environments: Protocol
to move Saccharina cultivation offshore
Chapter 2 Cultivation protocol for Saccharina latissima
Chapter 3 Derivation of clonal stock cultures and hybridization
of kelps: A tool for strain preservation and breeding
programs
Chapter 4 Cryopreservation of macroalgae
Chapter 5 Unraveling seaweeds bacteriomes: From field site
to computer screen
Chapter 6 Heavy metal ecotoxicity on the early life history
stages of macroalgae
Chapter 7 A simple protocol for a rapid and consistent
production of a large number of viable protoplasts
from the Ulvophycean species
Chapter 8 Purification of sporulation and swarming inhibitors
from Ulva: Application in algal life-cycle controlling
Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)
Section II: Chemical composition
Chapter 10 Biochar production from seaweeds
Chapter 11 Identification and quantification of laminarins
in brown algae
of macroalgae
Chapter 12 Determination of carbohydrate composition
Chapter 13 Quantification of proteins in seaweeds
Chapter 14 Comprehensive phytohormone quantification
in the red alga Pyropia yezoensis by liquid
chromatography-mass spectrometry
Chapter 15 Total phenolic content and antioxidant capacity
analysis of seaweed biomass
Chapter 16 Extraction of phycocyanin and phycoerythrin pigments
Chapter 17 Quantification and localization of reactive oxygen
species in marine macrophytes
Chapter 18 Metabolomics of intra- and extracellular metabolites
from micro- and macroalgae using GC-MS
and LC-MS
Chapter 19 Preparative extraction of exometabolites
from seaweed surfaces
Florian Weinberger
Chapter 20 Disruption-free solid-phase extraction of surface
metabolites from macroalgae
Emilio Cirri and Georg Pohnert
Section III: Cellular and molecular characterization
Chapter 21 The immunodetection and in situ imaging of
cell-wall polysaccharides in brown algae
Chapter 22 Atomic force microscopy based analysis of cell-wall
elasticity in macroalgae
Chapter 23 Dynamic and microscale mapping of cell growth:
Case of Ectocarpus filament cells
Chapter 24 Actin fluorescent staining in the filamentous brown
alga Ectocarpus siliculosus
Chapter 25 Cryofixation of brown algae for transmission
electron microscopy
Chapter 26 Probing the subcellular topography of
seaweeds: Transmission electron microscopy,
immunocytochemistry, and correlative light
microscopy
Chapter 27 Coralline algae preparation for scanning electron
microscopy and optical microscopy
Chapter 28 Extraction of high quality RNA from brown algae
for transcriptomic analysis
Chapter 29 Induction of sexual reproduction in Spirogyra
cultures for laser capture microdissection of gametes
and zygotes
Chapter 30 Cloning and expression strategies for the
postgenomic analysis of brown algae
Chapter 31 Polyethylene glycol-mediated transformation in
the green macroalga Ulva mutabilis (Chlorophyta):
A forward genetics approach
Index