SMC Proteins (eBook, PDF)

Methods and Protocols
Redaktion: Murayama, Yasuto; Kakui, Yasutaka

• Format: PDF

Produktbeschreibung

The aim of this volume is to provide a wide range of experimental methods for analyzing molecular functions of SMC complexes, such as microscopic imaging, protein localization, chromosome conformation analyses by deep DNA sequencing and biochemical reconstitution with purified proteins or cell lysate. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, SMC Proteins: Methods and Protocols aims to be comprehensive guide for researchers in the field.

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Produktdetails

• Verlag: Springer US
• Seitenzahl: 276
• Erscheinungstermin: 19. Oktober 2025
• Englisch
• ISBN-13: 9781071650059
• Artikelnr.: 75709465

Inhaltsangabe

Single-Molecule Analyses of Human Cohesin Complex.- Interaction between an Individual Cohesin Complex and DNA Revealed by Optical Tweezers and Single-Molecule Microscopy.- Visualising Binding of Single SMC Complexes to DNA Substrates Using Combinational Optical Tweezers and Fluorescence Microscopy.- Atomic Force Microscopy Analysis of Bridging-Induced Phase Separation Mediated by SMC Proteins.- Monitoring Local Chromatin Dynamics Regulated by SMC Complex in Fission Yeast.- DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology.- Gel Electrophoresis Analysis of SMC-Mediated Effects on DNA Topology.- The Use of the Homobifunctional Crosslinker BMOE to Investigate Cohesin Function by Measuring Protein-Protein and Protein-DNA Interactions.- Bulk FRET Analysis of the Conformation of the Cohesin Ring.- Assessing SMC Complex Function in Replication Fork Progression with DNA Fiber Assays.- Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation.- A Biochemical Assay to Monitor the Encounter between Cohesin and DNA Replication.- An In Vitro Assay to Study RSC Remodeling Activity Stimulation by the Cohesin Loader.- Purification and In Vitro Analysis of Escherichia Coli RecN.- Production and Purification of the Human SMC5/6 Core Complex from S. cerevisiae.- ChIA-PET Analysis for Investigating SMC-Mediated 3D Genomic Contacts.- High-Resolution Chromosome Conformation Capture Method to Study Archaeal SMC Complexes.