Techniques in Glycobiology (eBook, PDF)

Redaktion: Townsend, R. Reid; Hotchkiss, Arland T.

• Format: PDF

Produktbeschreibung

This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.

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Produktdetails

• Verlag: Taylor & Francis eBooks
• Seitenzahl: 648
• Erscheinungstermin: 25. Juni 1997
• Englisch
• ISBN-13: 9781482225860
• Artikelnr.: 57589931

Autorenporträt

R. Reid Townsend, Arland T. Hotchkiss

Inhaltsangabe

Mass spectrometry and NMR: new frontiers in nuclear magnetic resonance
spectroscopy - use of the Nano.NMR probe for the analysis of microgram
quantities of complex carbohydrates; analysis of anionic glycoconjugates by
delayed extraction matrix-assisted laser desorption time-of-flight mass
spectrometry. Glycolipids and the new physical methods: microscale analysis
of glycosphingolipids by thin-layer chromatography blotting;
high-performance liquid chromatography of gangliosides; a sensitive cell
suspension enzyme-linked immunosorbent assay measuring the density of cell
surface ganglioside and carbohydrate antigens. Protein 0-glycosylation:
effects of 0-glycosylation on protein structure - the role and
characterization of heavily 0-glycosylated mucin domains; predicting
mucin-type 0-glycosylation sites; experimental approaches to studying
0-glycosylation in vivo. Labelling oligosaccharides with chromophore and
fluorophores: highly sensitive pre-column derivation procedures for
quantitative determination of monosaccharides, sialic acids, and amino
sugar alcohols of glycoproteins by reversed phase high-performance liquid
chromatography; glycan labelling with the fluorophores 2-aminobenzamide and
anthranilic acid. Strategies for oligosaccharides separations and
sequencing: discovery and uses of novel glucosidases: resolution of
oligosaccharides in capillary electrophoresis; a comparison of
oligosaccharides profiling methods. Plant glycobiology: recent developments
in starch structure analysis using high-performance anion-exchange
chromatography with pulsed amperometric detection; red seaweed galactants -
methodology for the structural determination of corallinan, a different
agaroid. (Part contents)>