Tumor Immunology and Immunotherapy - Molecular Methods (eBook, ePUB)

• Format: ePub

Produktbeschreibung

Tumor Immunology and Immunotherapy - Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more. - Contains the authority of authors who are leaders in their field - Provides a comprehensive source on new methods and research in enzymology

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Produktdetails

• Verlag: Elsevier Science & Techn.
• Seitenzahl: 552
• Erscheinungstermin: 13. November 2019
• Englisch
• ISBN-13: 9780128186725
• Artikelnr.: 61681417

Inhaltsangabe

1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies



Sara Valpione and Luca Campana



2. Detection and quantification of cytosolic DNA



Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box



3. Methods to detect endogenous dsRNA induction and recognition



Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho



4. Quantification of eIF2alpha phosphorylation during immunogenic cell death



Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp



5. Assessment of annexin A1 release during immunogenic cell death



Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer



6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells



George R. Dubyak



7. Quinacrine-mediated detection of intracellular ATP



Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp



8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells



Francesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi



9. LEGENDplexT: Bead-assisted multiplex cytokine profiling by flow cytometry



Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun



10. Methods for measuring HMGB1 release during immunogenic cell death



Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer



11. Different measures of HMGB1 location in cancer immunology



Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze



12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues



Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast



13. Quantification of IDO1 enzyme activity in normal and malignant tissues



Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright



14. Enzyme activity of circulating CD73 in human serum



Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto and
Mariaelena Capone



15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays



Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg



16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis



Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik



17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization



Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris



18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1



Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao



19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies



Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata



20. High-throughput discovery of cancer-targeting TCRs



Lukas Bunse, Edward W. Green and Michael Platten



21. TCR validation toward gene therapy for cancer



Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten



22. Analysis of TCR ? CDR3 sequencing data for tracking anti-tumor immunity



Jiajia Zhang, Zhicheng Ji and Kellie N. Smith



23. Quantitative analysis of the T cell receptor repertoi